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首頁> 外文會議>Microfluidics, bioMEMS, and medical microsystems VIII >From Bioseparation to 'Artificial Micro Organs' - microfluidic chip based particle manipulation techniques
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From Bioseparation to 'Artificial Micro Organs' - microfluidic chip based particle manipulation techniques

機譯：從生物分離到“人工微器官”-基于微流控芯片的顆粒處理技術(shù)

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Microfluidic device technology provides unique physical phenomena which are not available in the macroscopic world.rnThese may be exploited towards a diverse array of applications in biotechnology and biomedicine ranging fromrnbioseparation of particulate samples to the assembly of cells into structures that resemble the smallest functional unit ofrnan organ.rnIn this paper a general overview of chip-based particle manipulation and separation is given. In the state of the artrnelectric, magnetic, optical and gravitational field effects are utilized. Also, mechanical obstacles often in combinationrnwith force fields and laminar flow are employed to achieve separation of particles or molecules.rnIn addition, three applications based on dielectrophoretic forces for particle manipulation in microfluidic systems arerndiscussed in more detail. Firstly, a virus assay is demonstrated. There, antibody-loaded microbeads are used to bind virusrnparticles from a sample and subsequently are accμmulated to form a pico-liter sized aggregate located at a predefinedrnposition in the chip thus enabling highly sensitive fluorescence detection.rnSecondly, subcellular fractionation of mitochondria from cell homogenate yields pure samples as was demonstrated byrnWestern Blot and 2D PAGE analysis. Robust long-term operation with complex cell homogenate samples while avoidingrnelectrode fouling is achieved by a set of dedicated technical means.rnFinally, a chip intended for the dielectrophoretic assembly of hepatocytes and endothelial cells into a structurernresembling a liver sinusoid is presented. Such "artificial micro organs" are envisioned as substance screening testrnsystems providing significantly higher predictability with respect to the in vivo response towards a substance under test.
機譯：微流體設(shè)備技術(shù)提供了宏觀世界所沒有的獨特的物理現(xiàn)象。這些可能會被廣泛應(yīng)用于生物技術(shù)和生物醫(yī)學(xué)領(lǐng)域，從微粒樣品的二糖分離到細(xì)胞組裝成類似于器官的最小功能單位的結(jié)構(gòu).rn本文概述了基于芯片的粒子處理和分離。在現(xiàn)有技術(shù)中，利用了電，磁，光和重力場效應(yīng)。而且，通常結(jié)合力場和層流來使用機械障礙來實現(xiàn)粒子或分子的分離。此外，更詳細(xì)地討論了基于介電電泳力在微流體系統(tǒng)中操縱粒子的三種應(yīng)用。首先，證明了病毒測定。在那里，負(fù)載抗體的微珠被用來結(jié)合樣品中的病毒顆粒，然后被聚集以形成皮升大小的聚集體，該聚集體位于芯片中預(yù)定的位置，從而實現(xiàn)高度靈敏的熒光檢測。第二，從細(xì)胞勻漿產(chǎn)量中對線粒體進行亞細(xì)胞分級分離純蛋白樣品通過Western Blot和2D PAGE分析證明。通過一套專門的技術(shù)手段，可以對復(fù)雜的細(xì)胞勻漿樣品進行穩(wěn)健的長期操作，同時避免電極結(jié)垢。最后，提出了一種芯片，用于將肝細(xì)胞和內(nèi)皮細(xì)胞介電泳組裝成類似于肝竇的結(jié)構(gòu)。這種“人工微器官”被設(shè)想為物質(zhì)篩選測試系統(tǒng)，該系統(tǒng)相對于對被測物質(zhì)的體內(nèi)反應(yīng)提供明顯更高的可預(yù)測性。

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