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首頁> 外文會議>Microfluidics, bioMEMS, and medical microsystems VIII >Development of an integrated microsystem for the multiplexed detection of breast cancer markers in serum using electrochemical immunosensors
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Development of an integrated microsystem for the multiplexed detection of breast cancer markers in serum using electrochemical immunosensors

機譯：使用電化學(xué)免疫傳感器對血清中乳腺癌標(biāo)志物進行多重檢測的集成微系統(tǒng)的開發(fā)

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摘要

A microsystem integrating electrochemical biosensoric detection for the simultaneous multiplexed detection of protein markers of breast cancer is reported. The immobilization of antibodies against each of carcinoembryonic antigen (CEA), prostate specific antigen (PSA) and cancer antigen 15-3 (CA15-3) was achieved via crosslinking to a bipodal dithiol chemisorbed on gold electrodes. This bipodal dithiol had the double function of eliminating non-specific binding and optimal spacing of the anchor antibodies for maximum accessibility to the target proteins. Storage conditions were optimized, demonstrating a long-term stability of the reporter conjugates jointly stored within a single reservoir in the microsystem. The final system has been optimized in terms of incubation times, temperatures and simultaneous, multiplexed detection of the protein markers was achieved in less than 10 minutes with less than ng/mL detection limits. The microsystem has been validated using real patient serum samples and excellent correlation with ELISA results obtained.
機譯：一個微系統(tǒng)集成電化學(xué)生物傳感檢測，用于乳腺癌蛋白標(biāo)記物的同時多重檢測。通過與金電極化學(xué)吸附的雙足二硫代二硫醇交聯(lián)，可以實現(xiàn)針對癌胚抗原（CEA），前列腺特異性抗原（PSA）和癌癥抗原15-3（CA15-3）的抗體固定化。該雙足二硫醇具有消除非特異性結(jié)合和錨定抗體最佳間隔的雙重功能，從而可以最大程度地接近目標(biāo)蛋白。優(yōu)化了存儲條件，證明了聯(lián)合存儲在微系統(tǒng)中單個存儲庫中的報道分子偶聯(lián)物的長期穩(wěn)定性。最終系統(tǒng)已在孵育時間，溫度和同時，不到10分鐘的時間內(nèi)以小于ng / mL的檢測限實現(xiàn)了蛋白質(zhì)標(biāo)記的多重檢測方面進行了優(yōu)化。該微系統(tǒng)已使用真實的患者血清樣品進行了驗證，并且與ELISA結(jié)果具有極好的相關(guān)性。

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